Peptide-modified PAMAM-based bone-targeting RNA delivery system

Patil S, Gao Y, et al.

Future Journal of Pharmaceutical Sciences

Northwestern Polytechnical University, Tangshan Normal University

RESEARCH SUMMARY

This study developed a bone-targeting RNA delivery system by modifying PAMAM dendrimers with an SDSSD peptide to selectively deliver antagomir-138-5p to osteoblasts. In an ovariectomized mouse model of osteoporosis, systemic administration of the RNA-loaded nanoparticles significantly increased bone mineral density, bone volume, and femoral mechanical strength. Three-point bending tests demonstrated higher fracture force, stress resistance, and elastic modulus in treated animals, confirming functional restoration of bone mechanical integrity. The results demonstrate the therapeutic potential of osteoblast-targeted RNA delivery for osteoporosis treatment.

Three-point bending tests were performed on excised mouse femurs using a CellScale UniVert mechanical testing system. Femurs were supported at fixed spans and loaded at the midpoint under displacement control until fracture. Load–displacement data collected during bending were used to calculate maximum force, stress, stiffness, and elastic modulus, enabling quantitative assessment of treatment-induced improvements in bone mechanical strength.

AUTHORS

Suryaji Patil, Yong-Guang Gao, Airong Qian.

PUBLICATION DETAILS

JOURNAL

Future Journal of Pharmaceutical Sciences

YEAR

2023

INSTITUTIONS

Northwestern Polytechnical University, Tangshan Normal University

COUNTRIES

China

INSTRUMENT USED

UniVert

TESTING METHODS

Flexural and Bending Testing

RESEARCH APPLICATIONS

Bone Tissue Engineering & MechanicsDrug Screening & Drug Delivery MechanicsInjectable & Regenerative BiomaterialsStem Cell Mechanobiology

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