PEER-REVIEWED PUBLICATION

2025

Microbial Living Materials Promote Coral Larval Settlement

Levy N, Kundu S, et al.

PNAS Nexus

University of Hawai'i, University of California – San Diego

RESEARCH SUMMARY
This study introduces a photopolymerizable bacterial reef ink (Brink) designed as a living material for enhancing coral larval settlement. Brink combines poly(ethylene glycol) diacrylate (PEGDA) and gelatin methacrylate (GelMA) to create a robust, biocompatible hydrogel matrix encapsulating two settlement-inducing bacterial strains—*Cellulophaga lytica* and *Thalassotalea euphylliae*. Coral settlement assays using *Montipora capitata* and *Pocillopora acuta* showed >5-fold and >4-fold settlement increases, respectively, compared with uncoated controls. The hydrogels exhibited tunable stiffness, high bacterial viability (>80% after 7 days), and optical transparency suitable for coral–bacteria interactions. Brink represents a scalable, sustainable biotechnological tool for accelerating coral recruitment in reef restoration and conservation.

CELLSCALE INSTRUMENT USED

MicroSquisher

The mechanical stiffness of Brink hydrogels was quantified using a CellScale MicroSquisher with 1 mm × 1 mm cylindrical samples compressed at 3 µm s⁻¹ up to 18% strain. The MicroSquisher provided high-resolution force–displacement data analyzed via MATLAB to determine Young’s modulus (~4.83 × 10⁵ Pa), confirming Brink’s 3.4× greater stiffness compared with PEGDA-only hydrogels and >100× greater than GelMA-only. These measurements established the material’s mechanical integrity for use as a bacterial encapsulation scaffold in dynamic marine environments.
AUTHORS

Natalie Levy, Samapti Kundu, Marnie Freckelton, Julie Dinasquet, Isabel Flores, Claudia T. Galindo-Martínez, Martin Tresguerres, Vanessa De La Garza, Yazhi Sun, Zahra Karimi, Crawford Drury, Christopher P. Jury, Joshua R. Hancock, Shaochen Chen, Michael G. Hadfield, R3D Consortium, Daniel Wangpraseurt.

PUBLICATION DETAILS
JOURNAL

PNAS Nexus

YEAR

2025

INSTITUTIONS

University of Hawai'i, University of California – San Diego

COUNTRIES

United States

INSTRUMENT USED

MicroSquisher

TESTING METHODS

Compression TestingHydrated and Temperature Controlled TestingMicro-Mechanical Testing

RESEARCH APPLICATIONS

3D Bioprinting & Bioink Materials TestingCell Laden HydrogelsHydrogel Mechanical TestingInjectable & Regenerative BiomaterialsScaffold Mechanical Testing

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