Mechano-induced cell metabolism disrupts the oxidative stress homeostasis of SAOS-2 osteosarcoma cells

Fanelli G, Alloisio G, et al.

Frontiers in Molecular Biosciences

University of Tuscia, University of Rome Tor Vergata

RESEARCH SUMMARY

Using LC-MS metabolomics and qPCR, the authors characterized SAOS-2 osteosarcoma cells exposed to cyclic uniaxial stretch (0.5% strain, 1 Hz, 24 h) in the MechanoCulture FX. Mechanical stimulation caused downregulation of glycolysis and TCA cycle pathways while enhancing glutaminolysis and disrupting the methionine cycle. This mechanical stress depleted intracellular glutathione, increased ROS levels, and altered expression of cytoprotective genes (SOD1, SIRT1, Nrf2), sensitizing cells to doxorubicin-induced death.

The MechanoCulture FX applied uniaxial cyclic stretch (0.5% elongation, 1 Hz, 24 h) to SAOS-2 osteosarcoma cells cultured on collagen-coated silicone wells. It enabled controlled, repeatable loading for metabolomic and gene-expression studies, validating mechanical induction of oxidative stress and drug sensitization.

AUTHORS

Fanelli G, Alloisio G, Lelli V, Marini S, Rinalducci S, Gioia M.

PUBLICATION DETAILS

JOURNAL

Frontiers in Molecular Biosciences

YEAR

2024

INSTITUTIONS

University of Tuscia, University of Rome Tor Vergata

COUNTRIES

Italy

INSTRUMENT USED

MechanoCulture FX

TESTING METHODS

Hydrated and Temperature Controlled TestingTensile TestingViscoelastic & Time-Dependent Testing

RESEARCH APPLICATIONS

Bone Tissue Engineering & MechanicsCancer MechanobiologyMechanotransductionStem Cell Mechanobiology

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