A Murine Experimental Model for the Mechanical Behavior of Viable Right-Ventricular Myocardium

This study established a novel murine experimental model for quantifying the multiaxial mechanical behavior of viable, full-thickness right-ventricular free wall myocardium. Using planar biaxial testing combined with detailed structural analysis of myofiber and collagen architecture, the authors demonstrated that murine RV myocardium exhibits strong mechanical anisotropy aligned with the apex-to-outflow-tract direction. Remarkably uniform transmural fiber organization was observed, closely corresponding to anisotropic stress–strain behavior. The study also introduced a complementary strain-energy–based framework to derive a representative average biomechanical response, providing foundational data for constitutive modeling of right-ventricular mechanics and remodeling.