Differences in the Mechanical Properties of Intestinal Tissue Based on Preservation Freezing Duration and Temperature

This study evaluates how freezing temperature and storage duration affect the mechanical integrity of intestinal tissue, with implications for tissue preservation, biomechanical testing, and translational research workflows. Using planar biaxial testing, the authors demonstrate that prolonged freezing and lower storage temperatures significantly alter stiffness and anisotropic mechanical behavior of porcine small intestine. The findings highlight the sensitivity of gastrointestinal tissue mechanics to preservation conditions and emphasize the importance of standardized handling protocols for biomechanical characterization.